Multi-Scale Computational Modeling of Bioprinted Tissue Constructs

Tissue engineering has been redefined as the use of
physical, chemical, biological and engineering
processes to control and direct the aggregate
behaviors of cells .This offers tremendous
opportunities for designing in vitro physiological
models to study disease pathogenesis, inventing
cell-based therapeutics in clinical applications and
developing novel pharmaceutical methods for reducing
the use of animals in drug testing. However, while
using biomanufacturing processes, cells are
subjected to an array of mechanical forces which may
injure it. While experimental studies have been
conducted for specific bio-fabrication systems to
understand cell responses, an engineering model is
also needed that can be used to predict the effect
of mechanical forces on cells. A multi scale
modeling approach for the analysis of cell damage in
bioprinted tissue constructs is presented here. The
approach includes analysis of the tissue constructs
at a macro scale model, developing a multi-cellular
scale model and a single cell model wherein the
microstructures of the cell like the nucleus and the
cytoplasm have been incorporated.